Comparison of the Level of Substance P and Neurokinin A in Gingival Crevicular Fluid of Sound and Symptomatic Carious Primary Teeth by ELISA
AbstractObjectives: Pulpal inflammation is often associated with odontogenic pain. Dental pulp is abundantly innervated with sensory fibers encompassing neuropeptides. Neurokinin A (NKA) and substance P (SP) are important neuropeptides in the dental pulp that can cause neurogenic inflammation. Since no previous study has assessed dental pulp neuropeptides in children, this study aimed to compare the level of NKA and SP in gingival crevicular fluid (GCF) of sound and symptomatic carious primary teeth.Materials and Methods: Samples of GCF were obtained of 20 sound and 20 painful carious primary teeth. Enzyme-linked immunosorbent assay (ELISA) was used to quantify neuropeptides in GCF. Data were analyzed using paired t-test, ANOVA, Kolmogorov-Smirnov test and correlation coefficient test.Results: A significant difference was noted in the level of NKA in GCF of painful and sound teeth (2.23 pg/ml in painful, and 1.84 pg/ml in sound teeth, P<0.05). The difference between the two groups regarding SP was not significant (2.23 pg/ml in painful, and 2.02 pg/ml in sound teeth, P>0.05).Conclusions: The results showed that the level of NKA and SP was higher in GCF of painful teeth compared to that of sound teeth. This difference was statistically significant with regard to NKA. Thus, these neuropeptides can serve as indicators for pathological activities in teeth with symptomatic irreversible pulpitis.
- Tickle M, Blinkhorn AS, Milsom KM. The occurrence of dental pain and extractions over a 3-year period in a cohort of children aged 3-6 years. J Public Health Dent. 2008 Spring;68(2):63-9.
- Caviedes-Bucheli J, Munoz HR, Azuero-Holguin MM, Ulate E. Neuropeptides in dental pulp: the silent protagonists. J Endod. 2008 Jul;34(7):773-88.
- Coutaux A, Adam F, Willer JC, Le Bars D. Hyperalgesia and allodynia: peripheral mechanisms. Joint Bone Spine. 2005 Oct;72(5):359-71.
- Sattari M, Ama-Ashari M, Hejazi M. Correlation between IgE and different states of dental pulps. Iran J Allergy Asthma Immunol. 2000;1(3):141-5.
- Sakallioglu EE, Lutfioglu M, Sakallioglu U, Ceylan GK, Pamuk F, Dede FO, et al. Gingival crevicular fluid levels of neuropeptides following dental restorations. J Appl Biomater Funct Mater. 2015 Jul 04;13(2):e186-93.
- Rechenberg DK, Galicia JC, Peters OA. Biological markers for pulpal inflammation: A systematic review. PLoS One. 2016;11(11):e0167289.
- El Karim IA, Lamey PJ, Linden GJ, Awawdeh LA, Lundy FT. Caries-induced changes in the expression of pulpal neuropeptide Y. Eur J Oral Sci. 2006 Apr;114(2):133-7.
- El Karim IA, Linden GJ, Irwin CR, Lundy FT. Neuropeptides regulate expression of angiogenic growth factors in human dental pulp fibroblasts. J Endod. 2009 Jun;35(6):829-33.
- Caviedes-Bucheli J, Correa-Ortiz JA, Ballestero AC, Jimenez M, Munoz A, Rivero C, et al. The effect of dentine-bonding agents on substance P release in human dental pulp. Int Endod J. 2010 Feb;43(2):95-101.
- Maggi CA. Tachykinins and calcitonin gene-related peptide (CGRP) as co-transmitters released from peripheral endings of sensory nerves. Prog Neurobiol. 1995 Jan;45(1):1-98.
- Awawdeh L, Lundy FT, Shaw C, Lamey PJ, Linden GJ, Kennedy JG. Quantitative analysis of substance P, neurokinin A and calcitonin gene-related peptide in pulp tissue from painful and healthy human teeth. Int Endod J. 2002 Jan;35(1):30-6.
- Caviedes-Bucheli J, Camargo-Beltran C, Gomez-la-Rotta AM, Moreno SC, Abello GC, Gonzalez-Escobar JM. Expression of calcitonin gene-related peptide (CGRP) in irreversible acute pulpitis. J Endod. 2004 Apr;30(4):201-4.
- Sattari M, Mozayeni MA, Matloob A, Mozayeni M, Javaheri HH. Substance P and CGRP expression in dental pulps with irreversible pulpitis. Aust Endod J. 2010 Aug;36(2):59-63.
- Caviedes-Bucheli J, Gutierrez-Guerra JE, Salazar F, Pichardo D, Moreno GC, Munoz HR. Substance P receptor expression in healthy and inflamed human pulp tissue. Int Endod J. 2007 Feb;40(2):106-11.
- Torabinejad M, Rotstein I. Endodontic-periodontic interrelationship, in: Torabinejad M, Walton RE, Fouad AF, (editors). Endodontics Principles and Practice. 5th ed. St. Louis: Saunders/Elsevier; 2015:107-109.
- Goodis H, Saeki K. Identification of bradykinin, substance P, and neurokinin A in human dental pulp. J Endod. 1997 Apr;23(4):201-4.
- Torabinejad M, Holland GR. The biology of dental pulp and periradicular tissues, in: Torabinejad M, Walton RE, Fouad AF, (editors). Endodontics Principles and Practice. 5th ed. St. Louis: Saunders/Elsevier; 2015:14-19.
- Rodd HD, Boissonade FM. Immunocytochemical investigation of neurovascular relationships in human tooth pulp. J Anat. 2003 Feb;202(2):195-203.
- Giannopoulou C, Mombelli A, Tsinidou K, Vasdekis V, Kamma J. Detection of gingival crevicular fluid cytokines in children and adolescents with and without fixed orthodontic appliancesActa Odontol Scand. 2008 Jun;66(3):169-73.
- Heidari A, Shahrabi M, Rokouei M, Amirzargar A, Rahbar P. Comparative study of substance P and neurokinin A in gingival crevicular fluid of healthy and painful carious permanent teeth. Dent Res J (Isfahan). 2017 Jan-Feb;14(1):57-61.
- Rodd HD, Boissonade FM. Substance P expression in human tooth pulp in relation to caries and pain experience. Eur J Oral Sci. 2000 Dec;108(6):467-74.
- Goodis HE, Bowles WR, Hargreaves KM. Prostaglandin E2 enhances bradykinin-evoked iCGRP release in bovine dental pulp. J Dent Res. 2000 Aug;79(8):1604-7.
- Caviedes-Bucheli J, Lombana N, Azuero-Holguin MM, Munoz HR. Quantification of neuropeptides (calcitonin gene-related peptide, substance P, neurokinin A, neuropeptide Y and vasoactive intestinal polypeptide) expressed in healthy and inflamed human dental pulp. Int Endod J. 2006 May;39(5):394-400.
- Avellan NL, Sorsa T, Tervahartiala T, Forster C, Kemppainen P. Experimental tooth pain elevates substance P and matrix metalloproteinase-8 levels in human gingival crevice fluid. Acta Odontol Scand. 2008 Feb;66(1):18-22.
- Zanini M, Meyer E, Simon S. Pulp inflammation diagnosis from clinical to inflammatory mediators: A systematic review. J Endod. 2017 Jul;43(7):1033-51.