Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture

  • Meysam Ganjibakhsh PhD Student, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran; Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  • Pouyan Aminishakib Assistant Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Parvaneh Farzaneh Assistant Professor, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Abbas Karimi Assistant Professor, Craniomaxillofacial Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Seyed Abolhassan Shahzadeh Fazeli Associate Professor, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran; Department of Molecular and Cellular Biology, School of Basic Science and Advanced Technologies in Biology, University of Sciences and Culture, Tehran, Iran
  • Moones Rajabi Adjunct Assistant Professor, Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Ahmad Nasimian PhD Student, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran; Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • Fereshteh Baghaei Naini Associate Professor, Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Hedieh Rahmati Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Neda sadat Gohari Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Nazanin Mohebali Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Masoumeh Asadi Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Zahra Elyasi Gorji Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Mehrnaz Izadpanah PhD Student, Department of Applied Cell Science and Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Shiva Mohamadi Moghanjoghi Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
  • Sepideh Ashouri Researcher, Human and Animal Cell Bank, Iranian Biological Resource Center, ACECR, Tehran, Iran
Keywords: Carcinoma, Squamous Cell of Head and Neck, Primary Cell Culture, Mouth Neoplasms

Abstract

Objectives: Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer worldwide. It is known as the eighth most common cancer in men and as the fifth most common cancer in women. Cytogenetic and biochemical studies in recent decades have emphasized the necessity of providing an appropriate tool for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including oral cancer. Here, we explain the establishment process of five primary oral cancer cells derived from an Iranian population.Materials and Methods: The specimens were obtained from five oral cancer patients. Enzymatic, explant culture and magnetic-activated cell sorting (MACS) methods were used for cell isolation. After quality control tests, characterization and authentication of primary oral cancer cells were performed by short tandem repeats (STR) profiling, chromosome analysis, species identification, and monitoring the growth, morphology and the expression of CD326 and CD133 markers.Results: Five primary oral cancer cells were established from an Iranian population. The flow cytometry results showed that the isolated cells were positive for CD326 and CD133 markers. Furthermore, the cells were free from mycoplasma, bacterial and fungal contamination. No misidentified or cross-contaminated cells were detected by STR analysis.Conclusions: Human primary oral cancer cells provide an extremely useful platform for studying carcinogenesis pathways of oral cancer in Iranian population. They may be helpful in explaining the ethnic differences in cancer biology and the individuality in anticancer drug response in future studies.

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Published
2017-10-30
How to Cite
1.
Ganjibakhsh M, Aminishakib P, Farzaneh P, Karimi A, Shahzadeh Fazeli SA, Rajabi M, Nasimian A, Baghaei Naini F, Rahmati H, Gohari N sadat, Mohebali N, Asadi M, Elyasi Gorji Z, Izadpanah M, Mohamadi Moghanjoghi S, Ashouri S. Establishment and Characterization of Primary Cultures from Iranian Oral Squamous Cell Carcinoma Patients by Enzymatic Method and Explant Culture. jdt. 14(4):191-02.
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Original Article(s)