Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm

  • Sholeh Ghabraei Assistant Professor, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Behnam Bolhari Associate Professor, Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Mohammad Marvi Sabbagh Dentist, Private Practice, Tehran, Iran
  • Mahsa Sobhi Afshar Postgraduate Student, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Doxycycline, Metronidazole, Ciprofloxacin, Calcium Hydroxide, Chlorhexidine, Enterococcus Faecalis

Abstract

Objectives: The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)2) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus faecalis (EF) from the root canal system. Materials and Methods: Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5.25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)2 mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μm. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs). Results: The paste of Ca(OH)2 mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days. Conclusions: It seems that Ca(OH)2 mixed with 2% CHX is more potent than the TAP against EF biofilm.

Author Biographies

Sholeh Ghabraei, Assistant Professor, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Assistant Professor, Department of Endodontics
Behnam Bolhari, Associate Professor, Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Associate Professor, Department of Endodontics
Mohammad Marvi Sabbagh, Dentist, Private Practice, Tehran, Iran
Dentist
Mahsa Sobhi Afshar, Postgraduate Student, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Postgraduate student, Department of Endodontics

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Published
2018-06-13
How to Cite
1.
Ghabraei S, Bolhari B, Marvi Sabbagh M, Sobhi Afshar M. Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm. jdt. 15(3):151-60.
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Original Article(s)