A Model for Induction of Dysplasia in Hamster Mucosal Pouch

  • Sara Pourshahidi Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  • Elaheh Ghasemzadeh Hoseini Department of Oral and Maxillofacial Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  • Hooman Ebrahimi Oral Medicine Department, Dental Branch, Islamic Azad University, Tehran, Iran
  • Mojgan Alaeddini Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  • Shahroo Etemad-Moghadam Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  • Shiva Shirazian Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Precancerous conditions, 12-dimethylbenz(a)anthracene, Administration, Mucosal

Abstract

Induction of premalignant lesions in animal models is of high value for research purposes. This study aimed to induce dysplasia in hamster mucosal pouch for investigation of dysplastic lesions using dimethylbenz(a)anthracene. The buccal pouch of 10 hamsters was painted with dimethylbenz(a)anthracene for 10 weeks every other day. At 5 and 10 weeks, they underwent histopathological analysis. Clinically, there was no change until week 7; after which mucosal thickening occurred. Hamsters scarified at 5 weeks and 10 weeks demonstrated mild and moderate dysplasia, respectively. dimethylbenz(a)anthracene is a useful tool for inducing dysplastic lesions in the buccal pouch mucosa of hamsters.

References

Gaboardi F, Bordinazzo R, Galli L. The Nd:YAG laser and methylene blue staining in the diagnosis and treatment of premalignant vesical lesions and carcinoma in situ (CIS). International urology and nephrology 1989; 21(3): 289-97.

sudhakara Reddy.R RK. Photodynamic Therapy in oral Disease. International Journal of Biological & Medical research 2012; 3(2): 1857-83.

J.M.Nauta. The results of CO2 laser surgery in patients with oral leukoplakia :a 25 year follow up. Oral oncology 2004; 41(1): 31-7.

Michael B.Flynn MW. Use of Carbon Dioxide Laser for treatment of premalignant Lesions of the Oral Mucosa. Journal of Surgical Oncology 1988; 37: 232-4.

Horch HH, Gerlach KL, Schaefer HE. CO2 laser surgery of oral premalignant lesions. International journal of oral and maxillofacial surgery 1986; 15(1): 19-24.

Mahakian LM, Farwell DG, Zhang H, et al. Comparison of PET imaging with 64Cu-liposomes and 18F-FDG in the 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch model of oral dysplasia and squamous cell carcinoma. Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging 2014; 16(2): 284-92.

Manjunath KS. Lasers in the Management of oral pre-malignant lesions. international journal of scientific study 2015; 3(5): 183-7.

Sun Z, Sood S, Li N, et al. Chemoprevention of 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral carcinogenesis in hamster cheek pouch by topical application of a dual inhibitor of epidermal growth factor receptor (EGFR) and ErbB2 tyrosine kinases. Oral oncology 2008; 44(7): 652-7.

Chen YK, Lin LM. DMBA-induced hamster buccal pouch carcinoma and VX2-induced rabbit cancer as a model for human oral carcinogenesis. Expert review of anticancer therapy 2010; 10(9): 1485-96.

Nagini S, Kowshik J. The Hamster Buccal Pouch Model of Oral Carcinogenesis. Methods in molecular biology 2016; 1422: 341-50.

Shin DM, Gimenez IB, Lee JS, et al. Expression of epidermal growth factor receptor, polyamine levels, ornithine decarboxylase activity, micronuclei, and transglutaminase I in a 7,12-dimethylbenz(a)anthracene-induced hamster buccal pouch carcinogenesis model. Cancer research 1990; 50(8): 2505-10.

Chen YK, Hsue SS, Lin LM. Immunohistochemical demonstration of p63 in DMBA-induced hamster buccal pouch squamous cell carcinogenesis. Oral diseases 2003; 9(5): 235-40.

Low KP, Bhuvaneswari R, Thong PS, Bunte RM, Soo KC. Novel delivery of Chlorin e6 using anti-EGFR antibody tagged virosomes for fluorescence diagnosis of oral cancer in a hamster cheek pouch model. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2016; 83: 143-54.

de CMJS, de Oliveira SC, Reis Junior JA, et al. Effects of imiquimod and low-intensity laser (lambda660 nm) in chemically induced oral carcinomas in hamster buccal pouch mucosa. Lasers in medical science 2013; 28(3): 1017-24.

Wani MK, Yarber RH, Ahmed A, Hengesteg A, Robbins KT. Cancer induction in the DMBA hamster cheek pouch: a modified technique using a promoter. The Laryngoscope 2001; 111(2): 204-6.

Parihar A, Dube A, Gupta PK. Photodynamic treatment of oral squamous cell carcinoma in hamster cheek pouch model using chlorin p6-histamine conjugate. Photodiagnosis and photodynamic therapy 2013; 10(1): 79-86.

Shen L, Xu Q, Li P, Zhou G. Efficacy of krypton laser photodynamic therapy for oral mucosa dysplasia in 9,10-dimethyl-1,2-benzanthracene-treated hamsters. Oncology letters 2013; 6(5): 1358-62.

Salum FG, Cherubini K, de Figueiredo MA, Amado Mde O, Rodrigues ET, Yurgel LS. Histologic evaluation of effect of thiocyanate topical application on hamster cheek pouch. Clinical oral investigations 2006; 10(3): 211-5.

Brekke TD, Steele KA. Inbred or Outbred? Genetic Diversity in Laboratory Rodent Colonies. 2018; 8(2): 679-86.

de CMJS, Pinheiro AN, de Oliveira SC, et al. Influence of laser phototherapy (lambda660 nm) on the outcome of oral chemical carcinogenesis on the hamster cheek pouch model: histological study. Photomedicine and laser surgery 2011; 29(11): 741-5.

Published
2019-05-29
How to Cite
1.
Pourshahidi S, Ghasemzadeh Hoseini E, Ebrahimi H, Alaeddini M, Etemad-Moghadam S, Shirazian S. A Model for Induction of Dysplasia in Hamster Mucosal Pouch. Front Dent. 16(5):402-406.
Section
Short Communication