Evaluation of the Effect of Propolis Nanoparticles on Antimicrobial Properties and Shear Bond Strenght of Orthodontic Composite Bonded to Bovine Enamel
the present study was an attempt to investigate the effects of propolis nanoparticles (prpNPs) on antimicrobial property and shear bond strength(SBS) of orthodontic composite bonded to bovine enamel
Materials and Methods:
60 bovine teeth were randomly divided into five groups(n=12). prpNPs were prepared at concentrations of 0%(control), 1%, 2%, 5% and 10% in a Transbond XT composite and used to bond stainless steel brackets to the teeth. Universal Testing Machine was used to measure the SBS between the bracket and teeth. After debonding the brackets, ARI index on the bracket’s base was measured. In the microbial test, composites with afore-mentioned concentrations of prpNPs were cured in metal disks. The bacteria used in this study included Streptococcus mutans, Streptococcus sanguinis, And Lactobacillus acidophilus which Antimicrobial effects of prpNPs were investigated by anti-biofilm, DAD and Eluted Component tests.
the group with 10% of prpNPs showed the lowest SBS. The growth of colonies of S.mutans and S.sanguinis at all concentrations (except for the 1%) was significantly lower than control group. The growth of L.acidophilus colonies significantly reduced at 5% and 10% groups.Growth inhibition zone was developed at 2%, 5% and 10% groups for S.mutans and S.sanguinis. the lowest colonies of S.mutans and S.sanguinis at all concentrations was obtained in the 15th day. L.acidophilus colonies decreased significantly at all concentrations (except for the 1%) until 30th day.
Nanopropolis has a significant antimicrobial effect at 2% and 5% concentrations, and the SBS is maintained within the acceptable clinical range at these concentrations.
Mansour AY, Drummond JL, Evans CA, Bakhsh Z. In vitro evaluation of self-etch bonding in orthodontics using cyclic fatigue. The Angle orthodontist. 2011;81(5):783-7.
Retief DH, Dreyer CJ, Gavron G. The direct bonding of orthodontic attachments to teeth by means of an epoxy resin adhesive. American journal of orthodontics. 1970;58(1):21-40.
Newman GV. Epoxy adhesives for orthodontic attachments: progress report. American journal of orthodontics. 1965;51(12):901-12.
van Waveren Hogervorst WL, Feilzer AJ, Prahl-Andersen B. The air-abrasion technique versus the conventional acid-etching technique: A quantification of surface enamel loss and a comparison of shear bond strength. American Journal of Orthodontics and Dentofacial Orthopedics. 2000;117(1):20-6.
Dalaie K, Mirfasihi A, Eskandarion S, Kabiri S. Effect of bracket base design on shear bond strength to feldspathic porcelain. European journal of dentistry. 2016;10(3):351-5.
Ng'ang'a PM, Ogaard B, Cruz R, Chindia ML, Aasrum E. Tensile strength of orthodontic brackets bonded directly to fluorotic and nonfluorotic teeth: an in vitro comparative study. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics. 1992;102(3):244-50.
Ahn SJ, Lim BS, Lee SJ. Surface characteristics of orthodontic adhesives and effects on streptococcal adhesion. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics. 2010;137(4):489-95; discussion 13A.
Cohen WJ, Wiltshire WA, Dawes C, Lavelle CL. Long-term in vitro fluoride release and rerelease from orthodontic bonding materials containing fluoride. American Journal of Orthodontics and Dentofacial Orthopedics. 2003;124(5):571-6.
Anderson AM, Kao E, Gladwin M, Benli O, Ngan P. The effects of argon laser irradiation on enamel decalcification: An in vivo study. American Journal of Orthodontics and Dentofacial Orthopedics. 2002;122(3):251-9.
Gorelick L, Geiger AM, Gwinnett AJ. Incidence of white spot formation after bonding and banding. American journal of orthodontics. 1982;81(2):93-8.
Ogaard B, Rolla G, Arends J. Orthodontic appliances and enamel demineralization. Part 1. Lesion development. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics. 1988;94(1):68-73.
Ogaard B, Rolla G, Arends J, ten Cate JM. Orthodontic appliances and enamel demineralization. Part 2. Prevention and treatment of lesions. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics. 1988;94(2):123-8.
Harazaki M, Hayakawa K, Fukui T, Isshiki Y, Powell LG. The Nd-YAG laser is useful in prevention of dental caries during orthodontic treatment. The Bulletin of Tokyo Dental College. 2001;42(2):79-86.
Gaines S, James T, Folan M, Baird A, O'Farrelly C. A novel spectrofluorometric microassay for Streptococcus mutans adherence to hydroxylapatite. Journal of microbiological methods. 2003;54(3):315-23.
Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta biomaterialia. 2008;4(3):707-16.
Zachrisson BU. Fluoride application procedures in orthodontic practice, current concepts. The Angle orthodontist. 1975;45(1):72-81.
Taylor R, Coulombe S, Otanicar T, Phelan P, Gunawan A, Lv W, et al. Small particles, big impacts: A review of the diverse applications of nanofluids. Journal of Applied Physics. 2013;113(1):011301.
Mitra SB, Wu D, Holmes BN. An application of nanotechnology in advanced dental materials. The Journal of the American Dental Association. 2003;134(10):1382-90.
Moreno MaIN, Isla MaI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology. 2000;71(1):109-14.
Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of ethnopharmacology. 1999;64(3):235-40.
Castaldo S, Capasso F. Propolis, an old remedy used in modern medicine. Fitoterapia. 2002;73(Supplement 1):S1-S6.
Ghisalberti EL. Propolis: A Review. Bee World. 1979;60(2):59-84.
Banskota AH, Tezuka Y, Kadota S. Recent progress in pharmacological research of propolis. Phytotherapy research : PTR. 2001;15(7):561-71.
Koo H, Gomes BP, Rosalen PL, Ambrosano GM, Park YK, Cury JA. In vitro antimicrobial activity of propolis and Arnica montana against oral pathogens. Archives of oral biology. 2000;45(2):141-8.
Rangasamy J, K Lakshmanan V, Chandrasekaran R, Kumar S, K S S, V. Nair S. In vitro Anti-cancerous and Anti-microbial Activity of Indian Propolis Nanoparticles2012.
Todd MA, Staley RN, Kanellis MJ, Donly KJ, Wefel JS. Effect of a fluoride varnish on demineralization adjacent to orthodontic brackets. American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics. 1999;116(2):159-67.
Ahn SJ, Lee SJ, Kook JK, Lim BS. Experimental antimicrobial orthodontic adhesives using nanofillers and silver nanoparticles. Dental materials : official publication of the Academy of Dental Materials. 2009;25(2):206-13.
Sodagar A, Sadegh Ahmad Akhoundi M, Bahador A, Farajzadeh Jalali Y, Behzadi Z, Elhaminejad F, et al. Effect of TiO2 nanoparticles incorporation on antibacterial properties and shear bond strength of dental composite used in Orthodontics2017. 67-74 p.
Aydin Sevinç B, Hanley L. Antibacterial activity of dental composites containing zinc oxide nanoparticles. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2010;94(1):22-31.
Hojati ST, Alaghemand H, Hamze F, Babaki FA, Rajab-Nia R, Rezvani MB, et al. Antibacterial, physical and mechanical properties of flowable resin composites containing zinc oxide nanoparticles. Dental Materials. 2013;29(5):495-505.
Kasraei S, Sami L, Hendi S, AliKhani M-Y, Rezaei-Soufi L, Khamverdi Z. Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restorative dentistry & endodontics. 2014;39(2):109-14.
Hernández-Sierra JF, Ruiz F, Pena DCC, Martínez-Gutiérrez F, Martínez AE, Guillén AdJP, et al. The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold. Nanomedicine: Nanotechnology, Biology and Medicine. 2008;4(3):237-40.
Ahn S-J, Lee S-J, Kook J-K, Lim B-S. Experimental antimicrobial orthodontic adhesives using nanofillers and silver nanoparticles. Dental Materials. 2009;25(2):206-13.
Akhavan A, Sodagar A, Mojtahedzadeh F, Sodagar K. Investigating the effect of incorporating nanosilver/nanohydroxyapatite particles on the shear bond strength of orthodontic adhesives. Acta odontologica Scandinavica. 2013;71(5):1038-42.
Grenho L, Barros J, Ferreira C, Santos VR, Monteiro FJ, Ferraz MP, et al. In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite. Biomedical materials. 2015;10(2):025004.
Reynolds IR, von Fraunhofer JA. Direct bonding of orthodontic brackets--a comparative study of adhesives. British journal of orthodontics. 1976;3(3):143-6.
Sharma S, Tandon P, Nagar A, Singh GP, Singh A, Chugh VK. A comparison of shear bond strength of orthodontic brackets bonded with four different orthodontic adhesives. Journal of Orthodontic Science. 2014;3(2):29-33.
Mirhashemi A, Bahador A, Kassaee M, Daryakenari G, Sadegh M, Akhoundi M, et al. Antimicrobial Effect of Nano-Zinc Oxide and Nano-Chitosan Particles in Dental Composite Used in Orthodontics ISMB TUMS2013. 1-10 p.
Poosti M, Ramazanzadeh B, Zebarjad M, Javadzadeh P, Naderinasab M, Shakeri MT. Shear bond strength and antibacterial effects of orthodontic composite containing TiO2 nanoparticles. The European Journal of Orthodontics. 2013;35(5):676-9.
Akhavan A, Sodagar A, Mojtahedzadeh F, Sodagar K. Investigating the effect of incorporating nanosilver/nanohydroxyapatite particles on the shear bond strength of orthodontic adhesives. Acta Odontologica Scandinavica. 2013;71(5):1038-42.
Fernandez L, Canut JA. In vitro comparison of the retention capacity of new aesthetic brackets. European journal of orthodontics. 1999;21(1):71-7.
Mirhashemi A, Bahador A, Kassaee M, Daryakenari G, Ahmad-Akhoundi M, Sodagar A. Antimicrobial Effect of Nano-Zinc Oxide and Nano-Chitosan Particles in Dental Composite Used in Orthodontics. Journal of Medical Bacteriology. 2015;2(3-4):1-10.
Sodagar A, Bahador A, Pourhajibagher M, Ahmadi B, Baghaeian P. Effect of Addition of Curcumin Nanoparticles on Antimicrobial Property and Shear Bond Strength of Orthodontic Composite to Bovine Enamel. Journal of Dentistry (Tehran, Iran). 2016;13(5):373-82.