The Influence of Antimicrobial Photodynamic Therapy Using Indocyanine Green-loaded Chitosan Nanoparticles Versus Biofilm Formation-related Gene Expression of Aggregatibacter Actinomycetemcomitans
Objective: Eradication of Aactinobacillus actinomycetemcomitans as an opportunistic periodontopathogen and inhibition of its pathogenic agents expression require to a new adjunctive therapeutic method. In this study, we described the outcome of the expression level of rcpA gene as a virulence factor associated with A. actinomycetemcomitans biofilm formation following treatment by antimicrobial photodynamic therapy (aPDT) using indocyanine green-loaded chitosan nanoparticles (CS-NPs@ICG).
Materials and Methods: CS-NPs@ICG was synthetized and examined with Scanning Electron Microscope (SEM). A. actinomycetemcomitans ATCC 33384 strain was treated by CS-NPs@ICG as a photosensitizer which was excited with a diode laser at the wavelength of 810 nm with energy density of 31.2 J/cm2. Quantitative real-time polymerase chain reaction was performed to determine the changes of rcpA gene expression level.
Results: Synthetized CS-NPs@ICG was confirmed via SEM image. The results of the current study revealed that CS-NPs@ICG- mediated aPDT could significantly decrease rcpA gene expression to 13.2-fold (P < 0.05). Also, there was a remarkable difference between aPDT using CS-NPs@ICG and ICG (P < 0.05). Based on the results, diode laser, ICG, and CS-NPs@ICG groups were not considerably able to down regulate the rcpA gene expression (P > 0.05).
Conclusions: In summary, the results showed that aPDT with CS-NPs@ICG leads to decrease the virulence factor of A. actinomycetemcomitans and can be used as a complementary therapy along with routine treatment for the successful of periodontitis therapy in vivo.
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