Pomegranate juice (punica granatum): a new storage medium for avulsed teeth.

  • Sara Tavassoli-Hojjati Assistant Professor, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran.
  • Elham Aliasghar Dentist, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran.
  • Fatemeh Ahmadian Babaki Assistant Professor, Department of Pharmacology and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Fatemeh Emadi Assistant Professor, Department of Pharmacology and Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Maliheh Parsa PhD Student, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Shohreh Tavajohi General Technician, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
  • Maryam Ahmadyar Dentist, Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Seyed Nasser Ostad Proffesor, Department of Toxicology & Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Keywords: Cell Culture, Neutral Red, Periodontal Ligament, Punica Granatum, Tooth Avulsion

Abstract

There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avulsed teeth was evaluated.PDL fibroblasts were obtained from healthy human premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultured cells were subjected to different concentrations of pomegranate juice (PJ), 1% Hank's balanced salt solution (HBSS) and tap water for 1, 3, 6 and 24 hours. PDL cell viability was assessed by the neutral red uptake assay.The results indicated that 7.5% PJ was the most effective solution for maintaining PDL cell viability amongst all the experimental solution's and time intervals (P<0.05). The results also showed that 1% PJ was as effective as HBSS for maintaining PDL cell viability. The amount of cell viability increased with increasing concentration of PJ at all time intervals (P<0.001). This effect is suggestive of the proliferative potential of PJ solution.In conclusion, PJ can be recommended as a suitable transport medium for avulsed teeth.
How to Cite
1.
Tavassoli-Hojjati S, Aliasghar E, Babaki FA, Emadi F, Parsa M, Tavajohi S, Ahmadyar M, Ostad SN. Pomegranate juice (punica granatum): a new storage medium for avulsed teeth. Front Dent. 11(2):225-32.
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