Total Antioxidant Capacity, Catalase Activity and Salivary Oxidative Parameters in Patients with Temporomandibular Disorders
Objectives: Temporomandibular disorders (TMD) are characterized by pain or discomfort in the temporomandibular joint, periauricular region, masticatory muscles, and neck on one or both sides. It may also be associated with joint sounds, restricted mandibular movements and mandibular deviation. Oxidative agents may have a deleterious role in the pathogenesis of joint diseases, and oxidative stress can lead to TMD. The aim of this study was to assess the oxidative stress biomarkers in the saliva of TMD patients and healthy controls.
Materials and Methods: This case-control study was conducted on 30 patients with TMDs (5 males and 25 females) with a mean age of 30.7±13.2 years, and 30 healthy controls (5 males and 25 females) with a mean age of 29.16±11.2 years. Saliva samples were collected according to the standard protocol and the total antioxidant capacity of the saliva (non-enzymatic), catalase activity, and malondialdehyde (MDA) levels were measured using the ferric reducing ability of plasma, Aebi’s method, and high-performance liquid chromatography, respectively. Finally, The MDA levels were analyzed by the Mann-Whitney test. Other quantitative parameters were analyzed by independent t-test.
Results: TMD patients had significantly higher salivary levels of MDA compared to the control group (P=0.001). But there were no significant differences in catalase (P=0.49) and total antioxidant capacity (P=0.22) of TMD patients and healthy controls.
Conclusion: It seems that oxidative stress may be involved in the pathogenesis of TMDs.
2. 2.Truelove EL, Sommers EE, LeResche L, Dworkin SF, Von Korff M. Clinical diagnostic criteria for TMD new classification permits multiple diagnoses. J Am Dent Assoc. 1992 Apr; 123(4):47-54.
3. Fernandes G, Siqueira JT, Gonçalves DA, Camparis CM. Association between painful temporomandibular disorders, sleep bruxism and tinnitus. Braz Oral Res. 2014; 28(1):1-7.
4. Milam SB, Zardeneta G, Schmitz JP. Oxidative stress and degenerative temporomandibular joint disease: a proposed hypothesis. J Oral Maxillofac Surg. 1998 Feb;56(2):214-23.
5. Etöz OA, Akçay H, Neşelioğlu S, Erel Ö, Alkan A. Total antioxidant capacity and total oxidant status of synovial fluids in patients with temporomandibular joint pain and dysfunction. Clin Oral Investig. 2012 Dec;16(6):1557-61.
6. Rodríguez de Sotillo D, Velly AM, Hadley M, Fricton JR. Evidence of oxidative stress in temporomandibular disorders: a pilot study. J Oral Rehabil. 2011 Oct;38(10):722-8.
7. Ozgocmen S, Ozyurt H, Sogut S, Akyol O, Ardicoglu O, Yildizhan H. Antioxidant status, lipid peroxidation and nitric oxide in fibromyalgia: etiologic and therapeutic concerns. Rheumatol Int.2006 May;26(7):598-603.
8. Canakci CF, Cicek Y, Yildirim A, Sezer U, Canakci V. Increased levels of 8-hydroxydeoxyguanosine and malondialdehyde and its relationship with antioxidant enzymes in saliva of periodontitis patients. Eur J Dent. 2009 Apr;3(2):100-6.
9. Demir CY, Kocak OF, Bozan N, Ersoz ME, Demir H. Is there a role for oxidative stress in temporomandibular joint disorders? J Oral Maxillofac Surg. 2018 Mar;76(3):515-20.
10. de Almeida C, Amenábar JM. Changes in the salivary oxidative status in individuals with temporomandibular disorders and pain. J Oral Biol Craniofac Res. 2016 Nov;6:S1-4.
11. Pabón A, Carmona J, Burgos LC, Blair S. Oxidative stress in patients with non-complicated malaria. Clin Biochem. 2003 Jan;36(1):71-8.
12. Richards RS, McGregor NR, Roberts TK. Association between oxidative damage markers and self-reported temporomandibular dysfunction symptoms in patients with chronic fatigue syndrome. J Chr Fatig Synd. 2004 Jan;12(3):45-61.
13. Draper HH, Csallany AS, Hadley M. Urinary aldehydes as indicators of lipid peroxidation in vivo. Free Radic Biol. 2000 Dec;29(11):1071-7.
14. Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymol. 1990 Jan;186:421-31.
15. Gomes P, Simão S, Lemos V, Amaral JS, Soares-da-Silva P. Loss of oxidative stress tolerance in hypertension is linked to reduced catalase activity and increased c-Jun NH 2-terminal kinase activation. Free RadicBiol Med. 2013 Mar;56:112-22.
16. Guo L, Shi W. Salivary biomarkers for caries risk assessment. J Calif Dent Assoc. 2013 Feb;41(2):107.
17. Nunes LA, Brenzikofer R, Macedo DV. Reference intervals for saliva analytes collected by a standardized method in a physically active population. Clin Biochem. 2011 Dec;44(17-18):1440-4.
18. Liu J, Duan Y. Saliva: A potential media for disease diagnostics and monitoring. Oral Oncol.
19. Aebi H. Catalase in Vitro. Methods Enzymol. 1984 Jan;105:121-6.
20. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem. 1996 Jul; 239(1):70-6.
21. Khoschsorur GA, Winklhofer-Roob BM, Rabl H, Auer T, Peng Z, Schaur RJ. Evaluation of a sensitive HPLC method for the determination of malondialdehyde, and application of the method to different biological materials. Chromatographia. 2000 Aug;52(3-4):181-4.
22. Dotan Y, Lichtenberg D, Pinchuk I. Lipid peroxidation cannot be used as a universal criterion of oxidative stress. Prog Lipid Res. 2004 May;43(3):200-27.
23. 23.Nitzan DW, Goldfarb A, Gati I, Kohen R. Changes in the reducing power of synovial fluid from temporomandibular joints with anchored disc. J Oral Maxillofac Surg. 2002 Jul;60(7):735-40.
24. Merry P, Winyard PG, Morris CJ, Grootveld M, Blake DR. Oxygen free radicals, inflammation, and synovitis: and synovitis: the current status. Ann Rheum Dis. 1989 Oct; 48(10):864.
25. Abu-Hilal M, McPhail MJ, Marchand L, Johnson CD. Malondialdehyde and superoxide dismutase as potential markers of severity in acute pancreatitis. JOP. 2006 Mar;7(2):185-92.
26. Holvoet P, Collen D, Van de Werf F. Malondialdehyde-modified LDL as a marker of acute coronary syndromes. JAMA. 1999 May; 281(18):1718-21.
27. Lawaf S, Azizi A, Tabarestani T. Comparison of Serum and Salivary Antioxidants in Patients with Temporomandibular Joint Disorders and Healthy Subjects. J Dent (Tehran). 2015 Apr; 12(4):263-70.
28. Lee MC, Kawai Y, Shoji H, Yoshino F, Miyazaki H, Kato H, et al. Evidence of reactive oxygen species generation in synovial fluid from patients with temporomandibular disease by electron spin resonance spectroscopy. Redox Rep. 2004;9(6):331-6.
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