Total Antioxidant Capacity, Catalase Activity and Salivary Oxidative Parameters in Patients with Temporomandibular Disorders

  • Neda Omidpanah Mail Department of Oral Medicine, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Saba Ebrahimi Private Practice, School of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Asad Vaisi Raygani Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran AND Department of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Hadi Mozafari Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Mansour Rezaei Department of Biostatistics, Kermanshah University of Medical Sciences, Kermanshah, Iran
Keywords:
Catalase, Malondialdehyde, Saliva, Temporomandibular Joint Disorders, Free Radicals, Oxidative Stress

Abstract

Objectives: Temporomandibular disorders (TMD) are characterized by pain or discomfort in the temporomandibular joint, periauricular region, masticatory muscles, and neck on one or both sides. It may also be associated with joint sounds, restricted mandibular movements and mandibular deviation. Oxidative agents may have a deleterious role in the pathogenesis of joint diseases, and oxidative stress can lead to TMD. The aim of this study was to assess the oxidative stress biomarkers in the saliva of TMD patients and healthy controls.
Materials and Methods: This case-control study was conducted on 30 patients with TMDs (5 males and 25 females) with a mean age of 30.7±13.2 years, and 30 healthy controls (5 males and 25 females) with a mean age of 29.16±11.2 years. Saliva samples were collected according to the standard protocol and the total antioxidant capacity of the saliva (non-enzymatic), catalase activity, and malondialdehyde (MDA) levels were measured using the ferric reducing ability of plasma, Aebi’s method, and high-performance liquid chromatography, respectively. Finally, The MDA levels were analyzed by the Mann-Whitney test. Other quantitative parameters were analyzed by independent t-test.
 Results: TMD patients had significantly higher salivary levels of MDA compared to the control group (P=0.001). But there were no significant differences in catalase (P=0.49) and total antioxidant capacity (P=0.22) of TMD patients and healthy controls.
Conclusion: It seems that oxidative stress may be involved in the pathogenesis of TMDs.

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Published
2020-09-13
How to Cite
1.
Omidpanah N, Ebrahimi S, Vaisi Raygani A, Mozafari H, Rezaei M. Total Antioxidant Capacity, Catalase Activity and Salivary Oxidative Parameters in Patients with Temporomandibular Disorders. Front Dent. 17.
Section
Original Article