Effect of Dietary Vitamin C on Orthodontic Tooth Movement in Rats

  • Amirfarhang Miresmaeili
  • Najmeh Mollaei
  • Ramin Azar
  • Nasrin Farhadian
  • Khosrou Mani Kashani
Ascorbic Acid, Orthodontics, Tooth Movement, Osteoclasts, Bone Remodeling


Objectives: Bone remodeling occurs during orthodontic treatment; this process enables tooth movement. Many factors can affect bone remodeling at the cellular level, such as nutritional supplements that can affect tooth movement. The present study was designed to evaluate the effect of dietary vitamin C on orthodontic tooth movement in rats. 

Materials and Methods: This study was carried out on 36 six-week male Wistar rats with a mean weight of 225+32 g, which were randomly allocated to two equal groups. Rats in the case group received 1wt% vitamin C in their daily water. Opening springs were placed on the incisor teeth of both case and control groups. After 17 days, rats were sacrificed; the distance between the mesio-incisal angles of these teeth was measured with a digital caliper. Histological sections were made containing incisor teeth and alveolar bone and stained by hematoxylin-eosin. The number of resorption lacunae was evaluated using light microscopy.

Results: Our findings showed that the amount of tooth movement in the vitamin C group was significantly higher than that in the control group (P<0.001). The osteoclast counts were significantly higher in vitamin C group (P=0.036).

Conclusion: Oral vitamin C can increase orthodontic tooth movement in rats with more osteoclast lacunae around root in the pressure area. 

How to Cite
Miresmaeili A, Mollaei N, Azar R, Farhadian N, Kashani KM. Effect of Dietary Vitamin C on Orthodontic Tooth Movement in Rats. Front Dent. 12(6):409-413.
Original Article